PFKFB3 Knockout cell line (THP-1)

PFKFB3 Knockout cell line (THP-1) Order

Catalog#

YKO-HT0524

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

PFKFB3 Knockout cell line (THP-1)

Species

Human

Gene Name

PFKFB3

Gene ID

5209

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript PFKFB3-203)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PFKFB3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PFKFB3 gene knockout PFKFB3 Knockout cell line (THP-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

PFKFB3 Knockout cell line (THP-1)

Catalog#

YKO-HT0524

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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