PFKFB3 Knockout cell line (THP-1)

location： Home > Products > KO Cell Lines > PFKFB3 Knockout cell line (THP-1)

PFKFB3 Knockout cell line (THP-1) Order

Catalog#

YKO-HT0524

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

PFKFB3 Knockout cell line (THP-1)

Species

Gene Name

PFKFB3

Gene ID

5209

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PFKFB3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PFKFB3 gene knockout PFKFB3 Knockout cell line (THP-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

PFKFB3 Knockout cell line (THP-1)

Catalog#

YKO-HT0524

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Subscribe Us

* E-mail：

Country/Region：

Name：

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.

PFKFB3 Knockout cell line (THP-1)

Ubigene